Chronic alcohol consumption enhances myeloid-derived suppressor cells (MDSC) in B16BL6 melanoma-bearing mice
Zhang, Hui H.
Meadows, Gary G.
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We previously found that chronic alcohol consumption decreases the survival of mice bearing subcutaneous B16BL6 melanoma. The underlying mechanism is still not completely understood. Antitumor T cell immune responses are important to inhibiting tumor progression and extending survival. Therefore, we examined the effects of chronic alcohol consumption on the functionality and regulation of these cells in C57BL/6 mice that chronically consumed 20% (w/v) alcohol and subsequently were inoculated subcutaneously with B16BL6 melanoma cells. Chronic alcohol consumption inhibited melanoma-induced memory T cell expansion and accelerated the decay of IFN-Γ producing T cells in the tumor bearing mice. Foxp3+CD4+CD25+ regulatory T cells were not affected; however, the percentage of myeloid-derived suppressor cells (MDSC) was significantly increased in the peripheral blood and spleen. T cell proliferation as determined by carboxyfluorescein succinimidyl ester (CFSE) labeling experiments in vitro was inhibited by alcohol consumption relative to control water drinking melanoma-bearing mice. Collectively, these data show that chronic alcohol consumption inhibits proliferation of memory T cells, accelerates the decay of IFN-Γ producing CD8+ T cells, and increases MDSC, all of which could be associated with melanoma progression and reduced survival.