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dc.creatorRatliff, Catherine
dc.date.accessioned2010-05-18T16:18:14Z
dc.date.available2010-05-18T16:18:14Z
dc.date.issued1999
dc.identifier.urihttp://hdl.handle.net/2376/2538
dc.description.abstractThe filamentous phage fd was converted to spheroidal shaped S-forms and studied by contact-mode atomic force microscopy under conditions of controlled differences in relative humidity coupled with changes in the applied tip force. Stable and reliable sample preparation was achieved by spin-coating freshly cleaved mica with particle containing solutions with very low salt content followed by humidity control. The filaments of fd form a plateau and then drop in height as increased force is applied [15]. The apparent height, however, of the S-forms decreased linearly as force increased, due to their nonrigid side chain packing. The findings that relative humidity plays a role on reliable and reproducible images is consistent with previously published results on other biological specimens.en_US
dc.description.sponsorshipHonors College, Washington State Universityen_US
dc.language.isoEnglishen_US
dc.rightsIn copyright
dc.rightsPublicly accessible
dc.rightsopenAccess
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.rights.urihttp://www.ndltd.org/standards/metadata
dc.rights.urihttp://purl.org/eprint/accessRights/OpenAccess
dc.subjectFilamentous phageen_US
dc.subjectDon Marvinen_US
dc.subjectDNAen_US
dc.subjectCloningen_US
dc.titleEffects of relative humidity and applied force on atomic force microscopy images of the S-form of phage fd
dc.typeElectronic Thesis or Dissertation
dc.description.citationRatliff, Catherine Effects of relative humidity and applied force on atomic force microscopy images of the S-form of phage fd, Fall 1999, Washington State University Honors College thesis, Fall 1999, 19 p.


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