Characterization of small RNAs derived from tomato spotted wilt virus infection by deep sequencing
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RNA silencing is a conserved eukaryotic surveillance mechanism thought to play a role in protection against invading nucleic acids such as viruses, transposons and transgenes. Virus infection leads to accumulation of viral small RNAs (vsRNAs) at high levels. The processing of vsRNAs can be from viral dsRNA replicative intermediates, self complementary regions of the viral genome or from the action RNA-dependent RNA polymerases on viral templates. The overall composition of the populations of vsRNAs generated by most plant viruses remains unknown. We have used deep sequencing techniques to characterize vsRNAs of Tomato spotted wilt virus (TSWV), a member of the genus Tospovirus, which causes economically important diseases in numerous crops in may parts of the world. The vsRNA profiles from TSWV-infected pepper, tomato plants were generated. Analysis of the vsRNas indicate multiple hot spots for small RNA production from the TSWV genome, the location of these hot spots are predominantly conserved across infections of different host species. Details of the origin, distribution and abundance of TSWVvRNAs in infected plant tissue were complied.