Evaluation of ion mobility mass spectrometry (IMMS) for determining the isomeric heterogeneity of bovine submaxillary mucin (BSM)
Hill, Herbert H. Jr.
MetadataShow full item record
Rapid separation and analysis of isomeric species are essential steps in the analytical protocol of carbohydrate structure analysis for glycomics research. This study evaluated the use of ion mobility spectrometry (IMS) prior to MS as an additional rapid isomer separation step prior to mass spectral analysis. Neutral oligosaccharide alditols of BSM were investigated in positive mode. Multiple isomers were detected for specific m/z on millisecond time scale, which would be difficult or impossible using MS alone or single LC column. Tandem MS provide valuable information for elucidating glycan structures and understanding the isomeric nature of glycans.