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dc.contributor.advisorKahn, Michael L
dc.creatorPrice, Jason
dc.date.accessioned2018-05-08T17:43:51Z
dc.date.available2018-05-08T17:43:51Z
dc.date.issued2017
dc.identifier.urihttp://hdl.handle.net/2376/13019
dc.descriptionThesis (Ph.D.), Molecular Biosciences, Washington State Universityen_US
dc.description.abstractRhizobia are soil bacteria that can associate with some legumes and participate in symbiotic nitrogen fixation. Bacterial CspA family members are small, single stranded nucleic acid binding proteins conserved throughout all domains of life. Here, the role of CspA family proteins in the symbiotic development of Sinorhizobium meliloti with Medicago sativa (alfalfa) is investigated. Expression and genetic deletion strain analysis revealed that CspA family proteins are differentially expressed in symbiosis and contribute to symbiotic effectiveness. RNAseq analysis of native co-immunoprecipitated RNAs identified a novel interaction between several CspA family proteins and the αR14 family of small non-coding RNA (sRNAs). Whole transcriptome analysis defined transcriptional defects associated with loss of CspA function. The development of a new in vitro RNA binding assay using broccoli, a Green Fluorescent Protein (GFP) RNA mimic, is described as well as its use in defining binding specificity of CspA family proteins with synthetic and native αR14 family sRNA structures. This work concludes that CspA family proteins interact with and influence the stability of specific RNA structures and these interactions control RNA regulated processes important for symbiotic development.en_US
dc.description.sponsorshipWashington State University, Molecular Biosciencesen_US
dc.languageEnglish
dc.rightsIn copyright
dc.rightsPublicly accessible
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.subjectBiochemistry
dc.subjectMicrobiology
dc.subjectEnvironmental science
dc.subjectbroccoli
dc.subjectCspA
dc.subjectmeliloti
dc.subjectRhizobia
dc.subjectsRNA
dc.subjectsymbiosis
dc.titleControl of RNA Structure by CspA Proteins in Rhizobia
dc.typeElectronic Thesis or Dissertation


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