WSU Vancouver University Scholars Honors Program Thesis
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Ozonation is a potential replacement for desalinated seawater purification by chlorination that produces carcinogenic byproducts. However, it can produce hypobromous acid that can become modified in biological systems to form bromotyrosine, simulating the body’s harmful immune response when not in the presence of a pathogen. Currently, 5 mg is considered the threshold for human consumption but this study identifies the specific dosage where rats begin to accumulate 3-bromotyrosine in their body. Observing the location of bromotyrosine within the thyroid, kidney and testes of the male Fischer 344 rats allows further indication of the methodology of tumor formation within each of the different regions. It creates a direct comparison between the three regions to know the sensitivities of the regions for tumor formation and the progression of cancer on a scale. It also followed up on previous research, to compliment other experiments that lack histological evidence to back their claims. To accomplish this, rats were dosed with bromine and bromate for seven days and then tissue samples from the rat’s thyroid, kidney and testes were taken to examine through staining with anti-3- bromotyrosine, at what threshold its presence was observed.