UNDERSTANDING AND CHARACTERIZING THE GENES ASSOCIATED WITH RPG1-MEDIATED RESISTANCE PATHWAY AGAINST STEM RUST
Gill, Upinder Singh
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Barley stem rust resistance gene, Rpg1 (Reaction to Puccinia graminis 1), confers durable resistance to barley cultivars against stem rust caused by many races of Puccinia graminis f. sp. tritici. The current study was focused on understanding and characterizing the genes associated with Rpg1 in barley. HvRIN4 (a barley homolog of RPM1 interacting protein in Arabidopsis), which interacts with RPG1 in an yeast two-hybrid system, was isolated, characterized and studied for its role in barley. Using gamma-ray mutagenesis, six mutants affecting the Rpg1-mediated resistance against stem rust were identified. All mutants retained a functional Rpg1 gene and were non-allelic, suggesting that the mutated genes were required for downstream or upstream signaling. Low and high-resolution genetic mapping of the mutant gamma 08-118 gene identified a 0.6 cM region of chromosome 6H of barley for its location. In another study the applicability of wheat Conserved Orthologous Set (COS) markers on diverse cereal germplasm and their efficacy was investigated. Overall, this research will help in understanding the mechanism of Rpg1¬-mediated durable resistance against stem rust in barley.