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dc.contributor.advisorPeace, Cameron
dc.creatorVerma, Sujeet
dc.date.accessioned2014-09-11T17:11:34Z
dc.date.available2014-09-11T17:11:34Z
dc.date.issued2014
dc.identifier.urihttp://hdl.handle.net/2376/5081
dc.descriptionThesis (Ph.D.), Department of Horticulture, Washington State Universityen_US
dc.description.abstractApple texture and flavor are fruit quality components that determine the success of new apple cultivars and consumer satisfaction. Better-tasting apples encourage greater consumption and health benefits. The combined suite of traits of apple acidity (tartness), crispness, and juiciness is here termed "fresh sensation". The Washington State University Apple Breeding Program (WABP) is dedicated to developing new apple cultivars with improved "fresh sensation" among other attributes, and seeks to apply trait-predictive DNA tests to optimize breeding efficiency. Co-localized quantitative trait loci (QTLs) were previously reported for "fresh sensation" traits at the Ma locus of linkage group (LG) 16 of the apple genome. Two major alleles, Ma and ma associated with high and low acidity were identified in studies of biparental populations. Recently, the RosBREED project established resources (such as reference germplasm sets phenotyped with standardized protocols and genotyped with genome-wide single nucleotide polymorphism [SNP] markers), tools (such as Pedigree-Based Analysis software), and approaches (such as the "Marker-Assisted Breeding Pipeline") to characterize "fresh sensation" QTLs in breeding germplasm and develop predictive DNA tests. QTL analyses must be conducted to confirm the presence of contrasting alleles in WABP germplasm. Determination of SNP-based haplotypes associated with QTL alleles, their effects, and their distribution in the RosBREED WABP breeding germplasm will provide DNA information to support parent crossing decisions. Besides the Ma locus, other loci with genetic variation may also exist. This study seeks to confirm the presence of contrasting alleles at the Ma locus in RosBREED WABP breeding germplasm, and other QTLs for acidity as components of "fresh sensation", to determine the effects of each allele. A genome-wide QTL analysis was conducted using FlexQTLen_US
dc.description.sponsorshipDepartment of Horticulture, Washington State Universityen_US
dc.language.isoEnglish
dc.rightsIn copyright
dc.rightsPublicly accessible
dc.rightsopenAccess
dc.rights.urihttp://rightsstatements.org/vocab/InC/1.0/
dc.rights.urihttp://www.ndltd.org/standards/metadata
dc.rights.urihttp://purl.org/eprint/accessRights/OpenAccess
dc.subjectGeneticsen_US
dc.subjectAgricultureen_US
dc.subjectHaplotypeen_US
dc.subjectmalic aciden_US
dc.subjectMarker-assisted breedingen_US
dc.subjectmarker-locus-traiten_US
dc.subjectPedigree-Based Analysisen_US
dc.subjectQuantitave trait locusen_US
dc.titleA PREDICTIVE GENETIC KNOWLEDGE FOR APPLE "FRESH SENSATION" PROVIDES INFORMATION TO INCREASE BREEDING EFFICIENCY IN WASHINGTON
dc.typeElectronic Thesis or Dissertation


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