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dc.creatorZhou, Yunyun
dc.creatorCall, Douglas R.
dc.creatorBroschat, Shira L.
dc.description.abstractThousands of whole-genome and whole-proteome sequences have been made available through advances in sequencing technology, and sequences of millions more organisms will become available in the coming years. This wealth of genetic information will provide numerous opportunities to enhance our understanding of these organisms including a greater understanding of relationships among species. Researchers have used 16S rRNA and other gene sequences to study the evolutionary origins of bacteria, but these strategies do not provide insight into the sharing of genes among bacteria via horizontal transfer. In this work we use an open source software program called pClust to cluster proteins from the complete proteomes of twelve species of Alphaproteobacteria and generate a dendrogram from the resulting orthologous protein clusters. We compare the results with dendrograms constructed using the 16S rRNA gene and multiple sequence alignment of seven housekeeping genes. Analysis of the whole proteomes of these pathogens grouped Rickettsia typhi with three other animal pathogens whereas conventional sequence analysis failed to group these pathogens together. We conclude that whole-proteome analysis can give insight into relationships among species beyond their phylogeny, perhaps reflecting the effects of horizontal gene transfer and potentially providing insight into the functions of shared genes by means of shared phenotypes.en_US
dc.rightsCreative Commons Attribution 4.0 International
dc.subjectHorizontal gene transfer
dc.titleWhole-proteome analysis of twelve species of Alphaproteobacteria links four pathogens
dc.description.versionPublished copy
dc.description.citationZhou, Y., D. R. Call, and S. L. Broschat, Whole-proteome analysis of twelve species of Alphaproteobacteria links four pathogens, Pathogens, Vol. 2, pp. 627-635, 2013. doi:10.3390/pathogens2040627.

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  • Broschat, Shira
    This collection features research and educational materials by Shira Broschat, Professor and Curriculum Coordinator for the School of Electrical Engineering and Computer Science at Washington State University.

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Creative Commons Attribution 4.0 International
Except where otherwise noted, this item's license is described as Creative Commons Attribution 4.0 International